OH-HARMONY-CAP: One Health Harmonisation of Protocols for the Detection of Foodborne Pathogens and AMR Determinants

Start: 1 January 2020
Duration: 3 Years
Domain: Integrative Activity
Key Words: One Health, harmonisation, capability, capacity, interoperability
Contact: Nadia Boisen (SSI)


OH-Harmony-CAP aims to collect information on current capabilities, capacities and interoperability at both the National Reference Laboratory (NRL) and the primary diagnostic level. The quantitative description of current and best practices and the development of harmonised protocols will identify and possibly close the gaps and suggest future studies of how best to detect and characterise food borne pathogens across the One Health sectors. A global strategic overview of laboratory capacity in the animal-food field, as provided by EULabCap, will be provided, updated and expanded.

The project will develop and test an OHLabCap survey at the NRL level in all EU/EEA countries followed by an adjusted OHLabCap survey of the primary diagnostic laboratories in countries that have been identified during the first survey. It will focus on six high priority bacteria, ten high priority parasites and AMR for Salmonella and Campylobacter. The project will also quantify current practices, and describe procedures and methods in the detection of foodborne pathogens and AMR for Salmonella and Campylobacter. Upon review, we will produce recommendations and guidelines on how to improve the quantitative data on foodborne pathogens. Focus will also be on selected bacteria and parasites. We will collect, analyse and rank current protocols according their ability to detect the model organisms Shiga toxin producing E. coli (STEC)/enterotoxin producing E. coli (ETEC) and Cryptosporidium.

Specific protocols of the highest quality will be designed to be tested, and will test the developed protocols in practical training seminars, and through E-learning, and include training in how to organise national networks and exercises in communication on both national and EU/EEA levels.


Gill, A., Dussault, F., McMahon, T., Petronella, N., Wang, X., Cebelinski, E., Scheutz, F., Weedmark, K., Blais, B., & Carrillo, C. (2022). Characterization of Atypical Shiga Toxin Gene Sequences and Description of Stx2j, a New Subtype. Journal of clinical microbiology. 60(3), e0222921. DOI: https://doi.org/10.1128/jcm.02229-21

Bai, X., Scheutz, F., Dahlgren, H. M., Hedenström, I., & Jernberg, C. (2021). Characterization of Clinical Escherichia coli Strains Producing a Novel Shiga Toxin 2 Subtype in Sweden and Denmark. Microorganisms. 9(11), 2374. DOI: https://doi.org/10.3390/microorganisms9112374

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