ToxSauQMRA

ToxSauQMRA: Study of the tropism and persistence of Toxoplasma gondii: from pork carcass to sausage and dry ham, a quantitative microbiological risk assessment

         Start: November 2019

         Duration: 3 years

         Research Domain: Foodborne Zoonoses

         Members: ANSES- France, RIVM- the Netherlands

         Contact: Dr Pascal Boireau (ANSES)

The Project

Toxoplasma gondii is an intracellular coccidian parasite and one of the most successful parasites worldwide. Sexual reproduction resulting in shedding of oocysts occurs only in felids (definitive hosts), but virtually all warm blooded animals can carry tissue cysts and act as intermediate hosts. Humans, as intermediate hosts, become infected with T. gondii through ingestion of oocysts (e.g. when handling soil or cat litter, or on unwashed vegetables) or tissue cysts in raw or undercooked meat. If a woman becomes infected for the first time during pregnancy, T. gondii is transmitted to the fetus in approximately 30% of the occasions (Thiebaut et al., 2007). This can result in abortion or a baby born with central nervous system abnormalities, chorioretinitis, unspecific signs, or without symptoms. T. gondii is also an important cause of disease in immune‐compromised individuals, and was a major cause of death in AIDS‐patients before the introduction of highly active retroviral therapy (Luft and Remington, 1992). Postnatal T. gondii infection has long been perceived as harmless, but is now recognized as an important cause of chorioretinitis for immunocompetent individuals (Gilbert and Stanford, 2000). Meat appears to be a major source of T. gondii infections in Europe, as in an European multi‐center case control study 30 to 63% of infections in pregnant women were attributed to meat, whereas 6 to 17% were most likely soil borne (Cook et al., 2000). Pigs, like other livestock, can harbour tissue cysts following the ingestion of oocysts or dead animals. The surveillance plan conducted by the Ministry of Agriculture of France in 2013, and carried out by ANSES in collaboration with URCA, showed that 3.0% (CI95-[0.9-5.0%]) of pigs raised in an in-door system and 6.3% (CI95-[2.6-9.9%]) of pigs raised outdoor were seropositive for T. gondii. This seroprevalence reached 13.5% (CI95-[13.1-13.9%]) in out-door breeding sows. Viable parasite could be isolated in 22% (25/113) and 47% (16/34) of the indoor and outdoor pig carcasses analysed respectively. With 40.4 kg per household in 2014, pork is the first meat consumed in France, whose ¾ is eaten in the form of sausage and salami products (FranceAgriMer, 2015). Among raw cured meats, dry sausage is the most widely consumed (about 75,000 tonnes of dry sausage per year in France). France alone produced just over 108,000 tonnes of sausages and dry sausages in 2015, representing about 9% of the total tonnage of all sausages (FICT data, 2016). The health safety of these products is therefore a major issue. The share played by the consumption of pork meat in human contaminations is not known, nor is the risk of transmission of the parasite via the consumption of cured meat products. As early as 1999, the Codex Alimentarius Commission recommended that microbiological risk assessment should be based on a quantitative estimate. The high prevalence of toxoplasmosis in France in humans (32%) and the fact that the main mode of contamination is food-borne, fully justifies the conduct of a quantitative microbiological risk assessment (QMRA). If, in France, we have the T. gondii seroprevalence in various meat-producing species (ovine, bovine, pig, horses) enabling such an analysis, it lacks however the quantitative estimates concerning the distribution of parasites in the various parts (muscles) of a carcass and the reduction of the parasite load according to the cooking, preparation or preservation, as identified by an expert group of ANSES in 2005 (AFSSA, 2005). The present project aims at answering this lack of knowledge, providing though the bases for a robust QMRA analysis in France, an European country with a high Toxoplasma positive rate among humans.

Filip Dámek

About me: I am 26 years old and I have been amazed by science and nature all my life. Therefore, when the time had come to decide what I’d like to do in the future, I applied to the University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic. Throughout the studies, I have built an affection towards the field of parasitology which resulted in my involvement in ongoing Toxoplasma gondii research . more than three years ago. Alongside the study, I had the opportunity to work at several prestigious institutes and I managed to acquire research knowledge in the laboratory and the terrain.

What motivated me to do a PhD: Among other things, I see the doctoral study as a great way to further deepen my knowledge and understanding of Toxoplasma gondii and moreover to contribute to the scientific progress in that matter. I am constantly looking forward to new challenges in the research and ways to overcome them. I consider myself open-minded as past experiences have taught me the importance of teamwork and establishing valuable connections for future scientific collaboration. I am fortunate to work on the OHEJP project, that has a great potential to better the animal health, thereby raising the quality of life for all of us.

News

Filip Dámek has been selected for this exciting PhD project! Welcome to the One Health EJP consortium!

Presentations

Filip Dámek has been selected for this exciting PhD project! Welcome to the One Health EJP consortium!

Publications

Filip Dámek has been selected for this exciting PhD project! Welcome to the One Health EJP consortium!

wso shellkadına özelkadın newsbacklink albacklink satışinstagram takipçi hilesimeme küçültmewso shellworld newstatto girlshacklink seoseo analysismakale programıbacklink salessite analysispubg hile alseo sorgulamahacklink panelseo analysisseo sorgulagoogle newshacklink satışbacklinkbacklink satışseo araclarıseo analizsite analizAntalya Escortwebroot shellbypass shellr57 shelladana escortcanlı bahisadana eskortnevşehir escortbedava bonus veren bahis siteleribedava bonusdeneme bonusu30 tl bonus veren bahis sitelerihoşgeldin bonusuhoşgeldin bonusu veren bahis siteleribahis siteleribahiskaçak iddaacanlı bahisTürkçe Bahis Şirketlerisex hikayeleriporno hikayelerkayseri escortpendik escortsex hikayependik escorttuzla escortpendik escortkurtkoy escortkartal escortapartman kamera sistemikamera sistemimaltepe escortporno izleporno izleümraniye escortizmit escort bayankocaeli escort bayanizmit escortc99 shellc99 shellhtml kod şifrelemeseo newshacklinkbacklink alçorlu escortçerkezköy escortsivas escortmanisa escortizmir escorthatay escorterzurum escortbalikesir escorturfa escorttrabzon escorttekirdag escortsamsun escortadana escortankara escortantep escortaydin escortbursa escortdenizli escortsakarya escortordu escortmugla escortdiyarbakir escorteskisehir escortkayseri escortkocaeli escortkonya escortmalatya escortmaras escortmardin escortmersin escortizmir escortankara escortmersin escorteskişehir escortgaziantep escortsakarya escortkayseri escortantalya escortistanbul escortbursa escortadana escortwso shellsite hız testiadıyamanescortafyon escortağrı escortaksaray escortamasya escortardahan escortartvin escortaydın escortbalıkesir escortbartın escortbatman escortbayburt escortbilecik escortbingöl escortbitlis escortçanakkale escortçankırı escortçorum escortdenizli escortdiyarbakır escortdüzce escortedirne escortelazığ escorterzincan escortbolu escortburdur escortgümüşhane escorthatay escortığdır escortısparta escortmaraş escortkarabük escortkars escortkastamonu escortkilis escortkırıkkale escortkırklareli escortkırşehir escortkocaeli escortkonya escortkütahya escortmalatya escortmanisa escortmardin escortmuğla escortmuş escortnevşehir escortniğde escortordu escortosmaniye escortrize escortsamsun escorturfa escortsiirt escortsinop escortşırnak escortsivas escorttekirdağ escorttokat escorttrabzon escorttunceli escortuşak escortvan escortyozgat escortyalova escortzonguldak escortkıbrıs escortpendik escortesenyurt escortzeytinburnu escortküçükçekmece escortbeşiktaş escorttuzla escortbeykoz escortfatih escortsancaktepe escortarnavutköy escortbağcılar escortümraniye escortbeyoğlu escortüsküdar escortesenler escortmecidiyeköy escortsarıyer escortetiler escortlevent escortbebek escortgaziosmanpaşa escortkartal escortbaşakşehir escortşişli escorthalkalı escortbahçelievler escortortaköy escorteyüp escortbahçeşehir escortkağıthane escorttaksim escortçekmeköy escortsultançiftliği escortşaşkınbakkal escortbayrampaşa escortbeylikdüzü escortkadıköy escortataşehir escortbakırköy escortmerter escortataköy escortnişantaşı escortkozyatağı escortavcılar escortbüyükçekmece escortkurtköy escortiçerenköy escortküçükyalı escortbostancı escortşirinevler escorterenköy escortadana escortadıyaman escortafyon escortağrı escortaksaray escortamasya escortankara escortantalya escortardahan escortartvin escortaydın escortbalıkesir escortbartın escortbatman escortbayburt escortbilecik escortbingöl escortbitlis escortbolu escortburdur escortbursa escortçanakkale escortçankırı escortçorum escortdenizli escortdiyarbakir escortdüzce escortedirne escortelazığ escorterzincan escorterzurum escorteskişehir escortantep escortgiresun escortgümüşhane escorthakkari escorthatay escortığdır escortistanbul escortizmir escortkarabük escortkaraman escortkars escortkastamonu escortkayseri escortkıbrıs escortkilis escortkırıkkale escortkırklareli escortkırşehir escortkocaeli escortkonya escortkütahya escortmalatya escortmanisa escortmaraş escortmardin escortmersin escortmuğla escortmuş escortnevşehir escortniğde escortordu escortosmaniye escortrize escortsakarya escortsamsun escortsiirt escortsinop escortmozrankGameloopporno izleçekiliş sitesihtml code encryptionserver scanwso shelloyun indirsunucu taramasite speed testinstagram takipçi hilesiWordpress Free Themeswordpress free temahtml kod şifrelemesunucu taramaTanıtım Yazısıwordpress themesseowordpress ücretsiz temaWho islogsuz shell indirlitespeed bypass shellindoxploit shellphp shellgoogle sıra buluculitespeed bypass shellbacklink sorgulamaeryaman escortistanbul bayan escortankara escorthacklink satışbacklink satışhacklink panelhacklinkerzurum escortgiresun escortbest stethoscoperaycon reviewsBest Dog Crateshapermint bra reviewswireless earbudsBest Gaming Monitorsviagra siparişlifta 20 mgcialis fiyatviagra 100 mg fiyatcialis jelviagra satın alcialis 5 mg fiyatviagra satın alviagra fiyatıdegravigrandedegra 100 mgorcafilcialis nedircialis 20 mg eczane fiyatıcialis fiyatcialis 20 mgcialis 100 mgviagra fiyatliftalifta 5 mghardciscialis eczanecialis 5 mg fiyatıcialis 100 mg fiyatcialis 5 mgviagra fiyatlarıviagra satışonline eczane viagraviagra eczanecialis 20 mgcialis 100 mgcialis hapcialis 20cialis eczanecialis satışcialis fiyatlarıcialis fiyatıviagra fiyatviagra fiyatlarıviagra eczaneviagra satın alpfizer viagra satışonline eczane viagrapfizer viagra satın alviagra siparişviagra sipariş hattıviagra fiyat 2021online viagra siparişviagra fiyat 2021 eczaneviagra fiyat eczanecialis 5 mgkamagra satın alkamagrakamagra jelkamagra jel siparişkamagra siparişkamagra fiyatkamagra jel fiyatıkamagra fiyatıkamagra 100mgkamagra 100mg fiyatsüper kamagrakamagra nedirSincan escortAnkara rus escortsultangazi escortbingöl escortsancaktepe escorteskişehir escortkırklareli escortçekmeköy escortbodrum escortkuşadası escortc99 shellc99 shelllifta 20 mgliftalifta 20lifta 20 mg nedirlifta nedirhacklink seokuşadası escortbeylikdüzü escortkarşıyaka escortgöztepe escortbornova escortçeşme escortbuca escortalsancak escortkonak escort