Q. Do you work in human or animal health sectors on laboratory diagnostics?
Q. Are you interested in metagenomics sequencing for detecting antimicrobial resistance (AMR) in pathogens?
One Health EJP FARMED Project successfully tested and showed that long-read metagenomics using the Oxford Nanopore technology MinION system for pathogen and AMR detection is feasible. This project was led by the Animal and Plant Health Agency.
Some FARMED project outputs and outcomes include:
- Creation of harmonised protocols for on-site metagenomic DNA extraction and sequencing library preparation on the MinION technology.
- Development of efficient mapping strategies that can identify the origin of the genetic context of AMR genes and identify the host bacterial species to enable specific pathogen detection.
The methodologies and tools developed by FARMED are highly applicable to a wide range of users to rapidly monitor AMR present in pathogens in many different environments.
For more information about these project outputs and their applications, see the FARMED webpage.